First check and update any existing Python installations:
sudo apt-get install
sudo apt-get update
sudo apt-get -y upgrade
Check which version of Python3 you have:
python3 -v
If you do not have python3, please install using:
sudo apt-get install python3
#Install pip
pip is a package manager for python. To install:
sudo apt-get install -y python3-pip
Open Terminal and type:
pip3 install [modulename]
for example:
pip3 install biopython
will install Biopython.
Please repeat and use pip3 to install all the required modules for ciderseq (see Readme)
- Download Muscle from:www.drive5.com/muscle/downloads.htm
- Extract the file and copy to your cider-seq folder.
- Rename the file to 'muscle'
- If placed in another folder, make sure you edit the path address in the
ciderseq_config.jsonfile.
- Download the latest blast file from: ftp://ftp.ncbi.nlm.nih.gov/blast/executables/blast+/LATEST/
- Extract the tar.gz file to any location on your machine:
tar -xzvf [blast filename].tar.gz -C [/target/directory]
- Export the BLAST executables to your path by using the following commands:
export PATH=$PATH:[/target/directory]